Course Code: 5.5.3
Semester: 5th Semester
Course Hours: 2 Theory
ECTS: 3
LEARNING RESULTS
Upon completion of the course, the student will:
- knows modern topics of molecular biology and genetics of the grapevine, of particular importance for the viticultural sector, such as the identification of varieties with molecular markers, clonal selection and genetic improvement of the grapevine.
- At the same time, the course provides all the basic knowledge required for the participation of the Department’s graduates in Programs
- Postgraduate Studies that require undergraduate knowledge of Molecular Biology and Genetics.
General Skills
- Search, analysis and synthesis of data and information, also using new technologies
- Adaptation to new situations
- Decision making
- Autonomous work
- Teamwork
- Exercise criticism and self-criticism
- Promotion of free, creative and inductive thinking
COURSE CONTENT
- Introduction to the methods of molecular biology and plant genetics
- The grapevine genome – nucleotide sequencing
- Construction of genomic and cDNA libraries
- Analysis of gene expression – PCR, DNA microarrays
- Developmental molecular biology of grapevine
- Tissue culture – cell culture techniques
- Production of healthy propagating material
- Genetic transformation
- Varietal identification methods
- Capturing genetic diversity
- Applications of molecular markers in grapevine improvement – clonal selection
- Biotechnology applications in disease diagnosis and pathogen detection
- Bioinformatics analysis of molecular/genetic data
- The course also includes lectures and study of contemporary scientific articles related to
- Vine Biotechnology.
- RECOMMENDED BIBLIOGRAPHY
Charalambidis K., Milioni D., Rigas S., Roussis A., Hatzopoulos P. Developmental molecular biology of plants. EMBRYO Publications, 2009
Hatzopoulos P. Plant Biotechnology, Embryo Publications, Athens 2001.
Acquaah G (2007) Principles of Plant Genetics and Breeding. Blackwell Publishing.
Conde C., Silva P., Fontes N., Dias A.P.C. et al. Biochemical changes throughout grape berry development and fruit and wine quality. Food, Global Science Books, 2007.
Gray D.J., Jayasankar S., Li Z.T. (2005) Vitis spp. Grape. In: Litz RE (ed) Biotechnology of fruit and nut crops, pp. 672–706.
Mullins M.G., Bouquet A. and Williams L.E. Biology of the Grapevine, Cambridge University Press, 1992.
Riaz A., Doligez R., Henry J., Walker M.A. (2007) Grape, in: Kole C. (Ed.), Genome Mapping and Molecular Breeding in Plants, Vol. 4, Fruits and Nuts, Springer-Verlag, Berlin, Heidelberg, pp. 63-101.
Roubelakis-Angelakis K. A. Molecular Biology & Biotechnology of the Grapevine. Kluwer Academic Publishers, 2nd edition, 2009.
Related scientific journals
- Plant Physiology and Biochemistry,
- Plant Science,
- Plant Physiology,
- Plant Molecular Biology,
- Genetics,
- Genome,
- Australian Journal of Grape and Wine Research,
- American Journal of Enology and Viticulture,
• Vitis – Journal of Grapevine Research