Course Tutors
Course code: 3.4
Semester: 3ο
Course category: Compulsory
Course hours: 2Theory + 2Lab
ECTS: 5
LEARNING RESULTS
After the end of the course students will be able to:
- have a comprehensive picture of the microbial biodiversity of grapes, musts and wines
- correlate the action of microorganisms to wine quality
- identify the microorganisms that carry out alcoholic, malolactic, acetic and other fermentations
- understand the biochemical capabilities and biological characteristics of wine microorganisms
- select and use yeasts and bacteria in wine production
- to carry out microbiological control on musts and wines.
General Skills
- Theoretical thinking and ability to transform theory into practice.
- Ability to apply knowledge in solving Wine & Beverage Microbiology problems.
- Ability to search, analyze and synthesize data and information from international literature and use the necessary technologies related to the presentation of research results.
- Acquiring the appropriate theoretical and practical knowledge background to enable further education at the level of postgraduate specialization studies and PhD.
- Search, analysis and synthesis of data and information, using the necessary technologies
- Adaptation to new situations
- Decision making
- Autonomous work
- Teamwork
- Respect for the natural environment
COURSE CONTENT
Theoretical part
- Microflora of grape, must and wine
- The development of yeasts during alcoholic fermentation. Metabolic pathways of respiration and fermentation.
- Metabolism and products of alcoholic fermentation.
- Factors affecting alcoholic fermentation.
- Sulfur metabolism in yeasts.
- Spontaneous and controlled fermentation.
- Bacteria in winemaking.
- Malolactic fermentation
- Microbial spoilage of wines.
Laboratory Part
- Introduction to Wine Microbiology. Laboratory safety
- Isolation of microorganisms from fermented wort
- Counting microorganisms in plates by the method of successive dilutions
- Microbial filtration – Microbiological control of wine
- Identification of microorganisms
- Evaluation of resistance of yeasts to sulfuric anhydride.
- Evaluation of the Killer character in yeasts of oenological interest
- Culture conditions and methods of separation of bacteria
- Practical practice in microbiological techniques
- Isolation of microorganisms from equipment, surfaces, air
- Assessment of knowledge, abilities and skills
Attending the workshops is mandatory
STUDENT EVALUATION
Final written exam including: Multiple choice test, short development questions and/or oral exam. Optional work.
RECOMMENDED BIBLIOGRAPHY
Greek
- Ilias Nerantzis, Panagiotis Tataridis, Maria Liuni, Vasilios Varelas. Wine Microbiology. (2015)
- Kapsopoulou’s notes. Wine Microbiology
Foreign language
- 2018. Compendium of International Methods of Analysis of Wines and Musts (2 vol.)
- Pascal Ribéreau-Gayon, Denis Dubourdieu, Bernard B. Donèche, Aline A. Lonvaud, Yves Glories, Alain Maujean, John Towey (Translator). Handbook of Enology, 2 Volume Set, 3rd Edition (2021)
- Helmut König, Gottfried Unden, Jürgen Fröhlich. Biology of Microorganisms on Grapes, in Must and in Wine Softcover reprint of the original 2nd ed. Springer (2017)
- C.Fugelsang : Wine Microbiology. Chapman & Hall (1997)
- Victoria Moreno, Arribas, M. Carmen Polo. Wine Chemistry and Biochemistry. Springer (2009)
- H.Fleet (ed.) : Wine Microbiology and Biotechnology. Harwood Academic Publishers (1993)
- Usseglio-Tomasset : Chimie oenologique. Technique et Documentation-Lavoisier (1995).
- Η.H.Dittrich, M. Grossmann : Mikrobiologie des Weines. Ulmer Verlag (2005).
- Delfini, Claudio,, Formica, Joseph V. Wine microbiology : science and technology. New York : Marcel Dekker, (2001)
- Lonvaud-Funel Aline, RENOUF Vincent, STREHAIANO Pierre. Microbiologie du vin. Lavoisier. (2010)
Scientific Journals
- American Journal of Enology & Viticulture
• Australian Journal of Grape and Wine Research
• VITIS – Journal of Grapevine Research
• OENO One -International Journal of Vine and Wine Sciences-Journal International des Sciences de la Vigne et du Vin
• Journal of Wine Research
• Journal of Wine Economics
• Bulletin de l’O.I.V. : Revue Internationale : Viticulture, Oenologie, Economie, Droit
• Revue des Οenologues